Important Aspects of Stem Cell Regenerative Science.

Our Orthopedic Specialists recommend Supplements to Turn on Telomerase. Any clinic that is involved with Stem Cells, PRP and Regenerative Medicine needs to consider the Telomeres or the ends of the DNA strand. Below, we see a diagram of the Telomere or end of the DNA strand. On the left we see an embryonic (young) cell with a long Telomere while on the right we see an adult (old) stem cell with a much shorter Telomere.


Telomere Diagram
The Telomeres are disposable buffers blocking the ends of the chromosomes. They are consumed during cell division meaning every time a cell reproduces it loses a small snippet of DNA. When the DNA reaches a certain critical length, the cell is programed to die. This explains the basis of most diseases and aging in general. A good practical example of this phenomenon concerns Dolly the Cloned sheep. Dolly was produced from an adult cell which was induced to reproduce. A new sheep was reproduced or cloned but Dolly died of old age at a young age because her DNA was old. If we can stem the tide on Telomere degradation we will dramatically increase stem cell effectiveness. There is an enzyme which eliminates Telomere damage and it is called Telomerase. The problem is that most cells have the ability to produce Telomerase but this ability is turned off or silenced and thus damage continues relentlessly. If we turn Telomerase back on or at least slow down the degradation, then stem cells will be more effective. We do know certain supplements can help achieve this goal. Our clinic prides itself in recommending these supplements. This may be one of the most important new frontiers in stem cell science and probably all of medicine.

 

Stem Cell Harvesting of Bone Marrow Cells and Fat Cells

We have solved the problem of small numbers of bone marrow derived mesenchymal stem cells by utilizing mesenchymal fat cells. What was one time thought of as a worthless tissue is a virtual gold mine in Regenerative Medicine.


 

Stem Cells in Fat VS Stem Cell in Bone Marrow

Adipose tissue is as important as bone marrow for like bone marrow aspirate it contains a cornucopia of regenerative cells. These cells include adipose-derived mesenchymal stem cells, hematopoietic stem cells, adipocytes (fat cells) and attached progenitor cells, T regulatory cells and monocytes, and perivascular cellular components. The following diagram shows types of cells obtained from fat.

 


Adipose Stem Cells
 

This may seem a bit technical but it is important to understand about these cells. The adipose derived mesenchymal stem cells exist in such high numbers that it is not necessary to grow these cells in a lab eliminating many of the problems associated with growing cells in the lab. Like other stem cells, these adipose stem cells reduce inflammation and cause cells to grow in number and turn into different types of tissues. While hematopoietic stem cells can accomplish tissue repair, the Adipose Stem Cells in the fat graft will die and release signals which stimulate other cells to become metabolically active. These metabolically active Stem Cells stimulate the microenvironment allowing for cellular regeneration. T regulatory cells are a component of the immune system that suppresses immune responses of other cells. This is an important “self-check” built into the immune system to prevent excessive reactions. Many experts in the stem cell field feel fat tissue may be the ideal regenerative tissue since it has everything we need including growth factors, regenerative cells, and the fat itself acts as a 3-d matrix. However, when we look at the science we realize that we need to use both bone marrow and fat to achieve the proper numbers of cells top accomplish repair. Fat is very rich in mesenchymal stem cells while it is somewhat lacking in hematopoietic stem cells. On the other hand we see that bone marrow is very rich in hematopoietic stem cells but as can be seen in the following diagram the numbers of mesenchymal stem cells diminishes with age in bone marrow. When one understands the science it is quite evident that we need to utilize both in order to accomplish repair.

 


number of mesenchymal stem cells as we age
 

Other Differentiators for the Institute of Regenerative Medicine’s Stem Cell Treatment Facility

Another major difference of our facility is the use of certain supplements. These supplements can significantly increase stem cell production in the body. This is done in a safe and efficient manner with the use of proprietary supplements. This is based on research performed at medical schools by world famous stem cell scientists (one of these facilities is the University Of South Florida School of Medicine). These supplements have essentially no side effects and can actually surpass medications which cost thousands of dollars and have some serious side effects. We take care of many professional athletes and it is not unusual for them to take between 50 and 100 supplement pills a day. We have noticed that when we harvest stem cells from patients who have been on our supplement regimen their yields are much higher even after a few weeks. The reason why we are so concerned with supplements is that they can have a profound effect on the stem cell niche (environment). In the diagram below we can see picture of a stem cell membrane. The membrane is the eyes and ears of the cell. It will interact with its environment. More information about supplements can be found in our supplement section.

 

Growth Factor Release from an Activated Platelet
 

Another aspect, which we are looking at, is the use of hyperbaric oxygen. We know that hyperbaric oxygen cans dramatically increase stem cell production. A huge misconception is that hyperbaric oxygen causes healing by increasing oxygen to the involved areas. Oxygen is actually toxic to the tissues in high doses. Hyperbaric oxygen will actually work by increasing a compound called Nitric Oxide (NO). What the Nitric Oxide does is stimulate a certain enzyme which dramatically increases the stem cell output from the bone marrow. It is the increased stem cells in the circulation that causes the beneficial effects of the hyperbaric oxygen not the oxygen. The stem cells are causing the repair. These concepts were presented by Dr. Thom at the University of Pennsylvania. One of our ideas is to use supplements which increase Nitric Oxide in the body. Interestingly enough we know that Viagra and Cialis will increase nitric oxide and thus probably stem cell output. We think that Nitric Oxide will be an increasingly important topic in stem cell medicine and PRP injections. Further discussion about this topic will be discussed in the near future.

 

The other fields which we feel are very important concern the use of light therapy and electrical current therapy. More can be learned about this in the sections on these entities. As one can see, the institute has tried to cover all bases. We have combined ideas and entities from different disciplines to create what we call our biological cocktail. We have combined conventional medicine with its foundation in the basic science of stem cells and platelets with alternative ideas such as supplements. Our treatment plan is a hybrid of a number of different successful approaches, which have caused tissues to heal when typically they did not in the past. We have taken these ideas and refined and improved them to create a far superior protocol than anything out there. Our success speaks for itself. We have performed well over 9000 cases and are still counting. We are approaching results of approximately 85% significant improvement in conditions treated. We do not rest on our laurels but strive to be the cutting edge of the cutting edge.